Open AccessIn vitro Photoprotective Evaluation and Development of Novel Nanoemulsion with Chromone Derivative
Author(s) -
Amanda S. Antunes,
Ana Paula Martins Gouveia,
Gabriela M. Diogo,
Jason G. Taylor,
Lucas Resende Dutra Sousa,
Tatiane Roquete Amparo,
Fernanda Barçante Perasoli,
Orlando David Henrique dos Santos,
Thiago Cazati,
Paula Melo de Abreu Vieira,
Ricardo Gonçalves Penido,
Viviane Martins Rebello dos Santos
Publication year - 2021
Publication title -
journal of the brazilian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.337
H-Index - 70
eISSN - 1678-4790
pISSN - 0103-5053
DOI - 10.21577/0103-5053.20210072
Subject(s) - chromone , chemistry , sulforhodamine b , absorbance , cytotoxicity , in vitro , nuclear chemistry , combinatorial chemistry , chromatography , stereochemistry , biochemistry
Chromone derivatives exhibiting high absorbance values in the UVA/UVB region were synthesized, and their photoprotective properties were evaluated. Chromones were prepared according to known literature procedures and characterized by high resolution mass spectrometry, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The in vitro solar protection factor (SPF) was determined by the Mansur method and cytotoxicity was evaluated using the sulforhodamine B assay. Two of the chromones synthesized demonstrated suitable SPF values and displayed no cytotoxic effect towards MRC-5 human fibroblasts at the tested concentrations, indicating great potential for future in vivo assays and clinical trials. Finally, the lead compound was incorporated into a nanoemulsion. Nanoemulsions showed high droplet size homogeneity and excellent stability. Chromones bearing methoxy substituents were found to be the most promising compounds with ideal photoprotective properties desirable for utilization and incorporation in sunscreen formulations.