Open AccessAllyl Dibenzoylmethane Derivative: Antimelanoma Activity and Study of Its Molecular Mechanism of Interaction with DNA
Author(s) -
Jefferson V. P. B. Baeta,
U. M. S. Andrade,
Rayane M. Oliveira,
Luís Guilherme de Oliveira,
Anésia A. Santos,
Gaspar Diaz-Muñoz,
M. S. Rocha,
Marisa Alves Nogueira Diaz
Publication year - 2021
Publication title -
journal of the brazilian chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.337
H-Index - 70
eISSN - 1678-4790
pISSN - 0103-5053
DOI - 10.21577/0103-5053.20210048
Subject(s) - dna , chemistry , helix (gastropod) , molecule , derivative (finance) , groove (engineering) , base pair , biophysics , stereochemistry , biochemistry , materials science , organic chemistry , biology , ecology , snail , financial economics , metallurgy , economics
In this study, we assessed the cytotoxic effect of 1,3-diphenyl-2-allyl-1,3-propanedione (DPAP) on B16F10 and Tm5 melanoma cells and investigated its interaction with DNA (deoxyribonucleic acid) using optical tweezers. The compound showed to be effective against B16F10 cells with selectivity index (SI) of 7.92 and interaction to the outer surface of the double helix, probably at the minor groove of DNA. This likely induced a bending deformation of the polymer chain, decreasing persistence length, and indicated that each DPAP molecule occupies a single base pair upon binding to the double helix.