Open AccessBILIARY PAH METABOLITES IN FISH FROM THE HIGHLY IMPACTED GUANABARA BAY, IN SOUTHEASTERN BRAZIL, DETERMINED BY FIXED AND SYNCHRONOUS FLUORESCENCE SPECTROSCOPY
Author(s) -
Marina Moreira Freire,
Cristina Gómez,
Anabela S. Oliveira,
Josino Costa Moreira,
Ana Rosa Linde Arias
Publication year - 2021
Publication title -
química nova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.214
H-Index - 73
eISSN - 1678-7064
pISSN - 0100-4042
DOI - 10.21577/0100-4042.20170823
Subject(s) - pyrene , bay , environmental chemistry , metabolite , contamination , naphthalene , fish <actinopterygii> , fluorescence spectroscopy , environmental science , chemistry , fluorescence , biology , ecology , fishery , oceanography , organic chemistry , geology , biochemistry , physics , quantum mechanics
Guanabara Bay (GB) covers the metropolitan region of the state of Rio de Janeiro in Brazil. GB is subject to heavy contamination by polycyclic aromatic hydrocarbons (PAHs), from intense oil activities, which pose an ecotoxicological threat. The aim of this study is to implement and optimize a fluorescence methodology for the determination of biliary PAH metabolites in fish species (burrfish and whitemouth croaker), in order to evaluate biliary PAH metabolites as a biomarker of exposure. Fish were sampled from GB and a control region. Naphthalene, pyrene and benzo(a)pyrene metabolites were determined by Fixed Fluorescence Method (FF), while 1-hydroxypyrene was assessed by Synchronous Fluorescence Spectroscopy (SFS). The implementation and optimization of the FF and SFS methods allowed the determination and evaluation of the exposure of these species to PAHs of pyrogenic and petrogenic origin. Biliary PAH metabolite determinations was proven to be a useful tool for environmental monitoring contamination assessments.