Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form | Zendy

D Sireesha | Zendy; Sai Lakshmi E | Zendy; E Sravya | Zendy; Vasudha Bakshi | Zendy

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Development and Validation of RP-HPLC Method for the Estimation of Sitagliptin Phosphate in Tablet Dosage Form

Author(s) -

D Sireesha,

Sai Lakshmi E,

E Sravya,

Vasudha Bakshi

Publication year - 2017

Publication title -

international journal of applied pharmaceutical sciences and research

Language(s) - English

Resource type - Journals

ISSN - 2455-8095

DOI - 10.21477/ijapsr.v2i3.8099

Subject(s) - chromatography , sitagliptin , sitagliptin phosphate , dosage form , retention time , elution , high performance liquid chromatography , phosphate buffered saline , volumetric flow rate , chromatographic separation , particle size , gradient elution , chemistry , methanol , linearity , materials science , medicine , insulin , physics , organic chemistry , metformin , quantum mechanics

A new simple, rapid, specific, accurate, precise and novel Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of Sitagliptin Phosphate in the pharmaceutical dosage form. The chromatographic separation for Sitagliptin was achieved with mobile phase containing methanol, Thermoscientific C18 column, (250x4.6 particle size of 5μ) at room temperature and UV detection at 248 nm. The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Sitagliptin was 1.91min. The above method was validated in terms of linearity, accuracy, precision, LOD and LOQ in accordance with ICH guidelines.

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