Open AccessMethod Development and Validation of RP-HPLC Method for the Estimation of Ormeloxifene
Author(s) -
Anusha Shivaraj,
Shireesha Battula
Publication year - 2017
Publication title -
international journal of applied pharmaceutical sciences and research
Language(s) - English
Resource type - Journals
ISSN - 2455-8095
DOI - 10.21477/ijapsr.v2i03.8253
Subject(s) - chromatography , linearity , robustness (evolution) , elution , high performance liquid chromatography , methanol , chromatographic separation , materials science , chemistry , electronic engineering , engineering , biochemistry , organic chemistry , gene
A new simple, specific, accurate, precise RP-HPLC method has been developed for the estimation of Ormeloxifene. The chromatographic separation for Ormeloxifene was achieved with mobile phase containing methanol :ACN(70:30 v/v), agilent C18 column (4.6 x150 mm) 5 μ at room temperature and UV detection at 274nm.The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Ormeloxifene was found to be 2.497min. The method was validated according to ICH guideline for linearity, specificity, precision, accuracy, LOD, LOQ and robustness in accordance with ICH guidelines.