Open Access
Genetic variability of cultivated plum (Prunus domestica L. & Prunus salicina Lindl.) in Morocco assessed by ISSR markers
Author(s) -
Youssef Ait Bella,
Said Bouda,
Youssef Khachtib,
Abdelmajid Haddioui
Publication year - 2021
Publication title -
australian journal of crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.304
H-Index - 44
eISSN - 1835-2693
pISSN - 1835-2707
DOI - 10.21475/ajcs.21.15.06.p3217
Subject(s) - prunus salicina , upgma , biology , genetic diversity , cultivar , dendrogram , prunus , horticulture , crop , genetic relationship , botany , genetic variability , microsatellite , genetic variation , genotype , agronomy , gene , allele , genetics , population , demography , sociology
Moroccan plum cultivars were genetically characterized for assessing diversity and relatedness. In this study, a total of 23 plum cultivars were analyzed using 14 Inter-Simple Sequence Repeat (ISSR) primers. A total of 100 bands were obtained, of which 84 were polymorphic (84%). The mean values of PIC, Rp, I and Ht were 0.45; 3.03; 0.42 and 0.27, respectively, implying the important genetic variability between the plum cultivars. Furthermore, the coefficient of genetic differentiation (GST) between all groups was 0.21 indicating that 21 % of total genetic variability was between groups and 79 % was within groups. The UPGMA dendrogram and Bayesian model-based clustering approach identified four gene pools of cultivars independently of their geographic origin and denomination. These results showed that the ISSRs markers can be a useful tool for detecting molecular polymorphism and to survey the genetic diversity in this fruit crop.