Open AccessVALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TELMISARTAN AND HYDROCHLOROTHIAZIDE CONTENT IN BULK AND PHARMACEUTICAL DOSAGE FORM
Author(s) -
Pramod Kumar,
AUTHOR_ID,
a Wuchen,
Lei Wang,
Zaheer Abbas,
AUTHOR_ID,
AUTHOR_ID,
AUTHOR_ID
Publication year - 2021
Publication title -
international journal of advanced research
Language(s) - English
Resource type - Journals
ISSN - 2320-5407
DOI - 10.21474/ijar01/13885
Subject(s) - hydrochlorothiazide , chromatography , telmisartan , dosage form , particle size , volumetric flow rate , linearity , chemistry , high performance liquid chromatography , materials science , analytical chemistry (journal) , medicine , engineering , electronic engineering , physics , quantum mechanics , blood pressure , radiology
A simple, accurate, precise and rapid stability indicating reverse phase High performance chromatography method was used for estimation of Telmisartan and Hydrochlorothiazide in bulk and fixed-dose combination solid oral dosage form. The proposed analytical method has been validated for specificity, Linearity, Accuracy, Precision and Robustness. The chromatography was achieved in a GL science, Inertsil C8 (Length 125x Diameter 4.0mm Particle size 5µm) column with gradient flow. The optimal chromatographic condition consisted of mobile phase pH 3.0 at a flow rate of 1.2mL/min, with a column temperature of 40°C, run time 14 minutes and detector wavelength of 270nm.