Open AccessOPTIMIZATION SEQUENTIAL MICROWAVE ULTRASONIC ASSISTED EXTRACTION TO EXTRACT POLYPHENOL FROM MORINGA OLEIFERA LEAVES USING MULTIRESPONSE SURFACE METHODOLOGY
Author(s) -
Gian Restu Prinanda,
Aji Prasetyaningrum,
Akhmad Mustofa Yannie Asrie Widanti Hangga Wahyudi,
Dwi Eni Purwati
Publication year - 2021
Publication title -
international journal of advanced research
Language(s) - English
Resource type - Journals
ISSN - 2320-5407
DOI - 10.21474/ijar01/12858
Subject(s) - moringa , maceration (sewage) , polyphenol , extraction (chemistry) , chromatography , chemistry , response surface methodology , yield (engineering) , solvent , antioxidant , materials science , food science , biochemistry , metallurgy , composite material
Moringa leaves contain phenolic compounds and flavonoids which are useful as antioxidants. The disadvantages of traditional extraction methods, such as maceration, include a lengthy extraction process and the use of excessive solvent. Therefore, in this study, moringa leaf extraction was carried out using sequential microwave ultrasonic-assisted extraction (MUAE) to speed up the extraction time, and get more yields. This study aimed to improve the extraction of Moringa leaves with MUAE by adjusting the extraction time, temperature, and ethanol percentage. The conditions varied at extraction time (0, 10, 15, 20, 30 minutes), temperature (30, 40, 50, 60, 80 oC), and ethanol concentration (0, 30, 50, 70, 90%). The findings revealed that the best conditions for extraction Moringa leaves with sequential microwave-ultrasonic assisted extraction were at a time of 15 minutes, a temperature of 55oC, and an ethanol percentage of 54%. In this condition, the extract yield was 12.95%, total phenolic 330.38 GAE mg / 100 g, flavonoids in total 298.15 QE mg / 100 g, the IC50for antioxidant activity is 78.37 ppm.