Open AccessANTI-CANCER POTENTIAL OF COPPER OXIDE NANOPARTICLES AGAINST MURINE MAMMARY ADENOCARCINOMA (AMN-3) CELLS
Author(s) -
Khalil A. A. Khalil,
Amer T. Tawfeeq,
Ghassan M. Sulaiman,
Marwa D. Jaaffer
Publication year - 2021
Publication title -
international journal of advanced research
Language(s) - English
Resource type - Journals
ISSN - 2320-5407
DOI - 10.21474/ijar01/12607
Subject(s) - acridine orange , cytotoxic t cell , apoptosis , ethidium bromide , chemistry , mtt assay , cytotoxicity , microbiology and biotechnology , cell culture , cancer cell , growth inhibition , biochemistry , biology , medicine , in vitro , cancer , dna , genetics
This study was designed to evaluate the cytotoxic effect of copper nanoparticles in murine adenocarcinoma cells (AMN-3). The exposure period of cell line was performed at 24 hr in a microtitration plate under complete sterile conditions. Different concentrations were used started from 0.39 μgmL-1to 50 μgmL-1 in three independent experiments. First, the cells were stained by MTT and the absorbance were measured using Elisa reader at 492 nm. The treatment with nanoparticles showed a significant inhibition (P< 0.05) on cells and the effect was concentration dependent, the highest inhibition was 88% at 50 μgmL-1, while was 15% at 0.39 μgmL-1 and the inhibitory concentration 50 was 1.5 μgmL-1. The cell death was evaluated in cell line after a treatment with CUNPs through two types of assessments, which were mitochondrial membrane potential assay and acridine orange- ethidium bromide dual staining assays. Results revealed that the tested substances showed a potent inhibitory cytotoxic effect against the proliferation of AMN-3 cells through apoptosis.