Open AccessIN VITRO SCREENING OF SIDEROPHORE-PRODUCERS FROM UPLAND RICE ROOT ENDOPHYTIC BACTERIA FOR ANTAGONISTIC ACTIVITIES AGAINST FUSARIUMOXYSPORUM, ANDXANTHOMONASORYZEA PV. ORYZAE
Author(s) -
Linh T. T. Ta,
Nga T. P.,
Huong Thi Mai To,
DongIl Chung,
Ha Hoang Chu,
Binh Thi Nguyen Le,
Phuong Nguyen
Publication year - 2020
Publication title -
international journal of advanced research
Language(s) - English
Resource type - Journals
ISSN - 2320-5407
DOI - 10.21474/ijar01/12179
Subject(s) - siderophore , biology , orange (colour) , crop , antifungal , horticulture , antimicrobial , population , bacteria , botany , microbiology and biotechnology , agronomy , genetics , demography , sociology
Rice is a staple crop for a large part of the world population including Vietnam. Although its export is ranged of second place over the world just after Thailand, rice production in Vietnam is challenging by various issues. The most significant is application of agrochemicals, which are not eco-friendly, leading to side effects on the ecosystem and human health. Endophytic bacteria (EB) possess different beneficial traits such as Plant Growth Promoting (PGP) and antagonistic activities against plant pathogens. In this study, a collection of 150 EB strains have been isolated from upland rice roots grown on the mountain-farm in the Tung village, Nam Co commune, Mu Cang Chai district, Yen Bai province, Vietnam. 119/150 isolates showed siderophoreproduction ability including 12 isolates showed the halo orange zone surrounding colonies of 1 – 10 mm in diameter (Dh), considering them as low level of siderophore production, 80 strains exhibited the moderate activity, Dh of 11 – 30 mm and 27 strains showed strong/high activity, Dh more than 30 mm. All 119 endophytic bacterial isolates were screened for antifungal activity against Fusariumoxysporum (Fo), and antibacterial activity against ​Xanthomonasoryzaepv.​oryzae (Xoo). Of them, thirty isolates showed either antifungal or antibacterial or both activities. Indeed, five isolates showed antifungal activity fifteen isolates exhibited the antibacterial activity. Especially, ten isolates named NC88, NC89, NC126, NC129, NC130, NC131, NC134, NC152, NC153, and NC156 showed antimicrobial activity against both tested pathogens. These results are the starting-up for further investigation in planta under conditions of net house and field trial in order to reveal the potential strains for the development of bioinoculants toward to control the diseases caused by Fo andXoo.