Open AccessTECHNICAL SHEET OF COMPARATIVE STUDY OF TWO TECHNIQUES CONTROL OF DNA FROM YEAST ISOLATES PRODUCING PECTINASE
Author(s) -
Coulibaly Wahauwouélé Hermann,
Bouatenin Koffi Maïzan Jean-Paul,
Kouamé Kohi Alfred,
Amoikon Tiemele Laurent Simon,
Sanogo Yahya Maimouna,
Akissi Dogbo Marius
Publication year - 2020
Publication title -
international journal of advanced research
Language(s) - English
Resource type - Journals
ISSN - 2320-5407
DOI - 10.21474/ijar01/11751
Subject(s) - pectinase , agarose gel electrophoresis , agarose , chromatography , dna extraction , polymerase chain reaction , dna , gel electrophoresis , yeast , electrophoresis , chemistry , microbiology and biotechnology , biology , biochemistry , enzyme , gene
Polymerase Chain Reaction (PCR) is a widely used technique in the field of molecular biology to rapidly make very large number of copies of a specific DNA sample for detailed studies. The success of the technique however is dependent on the on the quality, i.e purity of the extracted DNA specimen. The aim of this study was to evaluate the quality of extracted DNA from pectinase producing yeast to determine the suitability of the extraction method to produce pure extract without non-inhibiting substances.In this study, DNA extracts from six (06) isolates of pectinase-producing yeasts were quantitatively and qualitatively analyzed using NanoDrop spectrophotometry and agarose gel electrophoresis methods. These analyses showed that the concentration of DNA extracts from the isolates evaluated by the NanoDrop spectrophotometric method ranged from 403.8 to 1082.4 ng/µL and the purity index A 260/280 was between 2.03 and 2.11. In sum, agarose gel electrophoresis showed that the intensity of the DNA bands was irregular and not necessarily in line with the data provided by the NanoDrop spectrophotometry.