Open AccessGlial and endothelial blood-retinal barrier responses to amyloid-β in the neural retina of the rat
Author(s) -
Peter J. Anderson,
Helena Watts,
Christopher J. Hille,
Karen L. Philpott,
Peter Clark,
M Croucher S Gentleman,
L.S. Jen
Publication year - 2008
Publication title -
clinical ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.025
H-Index - 56
eISSN - 1177-5483
pISSN - 1177-5467
DOI - 10.2147/opth.s3967
Subject(s) - nestin , glial fibrillary acidic protein , retinal , retina , blood–retinal barrier , amyloid beta , microbiology and biotechnology , neural stem cell , chemistry , medicine , pathology , neuroscience , biology , ophthalmology , endocrinology , immunohistochemistry , stem cell , diabetic retinopathy , disease , diabetes mellitus
The effects of an intravitreal or subretinal injection of soluble or aggregated forms of Abeta(1-42) on retinal nestin-immunoreactivity (-IR) and glial fibrillary acidic protein (GFAP)-IR in astrocytes and Müller glial cells and the integrity of the blood-retinal barrier (BRB) were tested in the in vivo rat vitreal-retinal model. Retinas were exposed for 1, 2, 3, 5 or 30 days. We present novel data demonstrating that aggregated Abeta(1-42) up-regulates nestin-IR in astrocytes and Müller cells, with a graded response directly related to the length of pre-injection aggregation time. Similar results were obtained with GFAP-IR, but the signal was weaker. An intravitreal injection of aggregated Abeta(1-42) led to VEGF-IR up-regulation, particularly in the GCL and to a lesser extent in the INL. VEGFR1-IR (Flt1) was also increased, particularly in Müller cells and this was accompanied by marked leakage of albumin into the retinal parenchyma of the injected eye, but not in the contralateral eye.