Open AccessHeme oxygenase-1 induction in the brain during lipopolysaccharide-induced acute inflammation
Author(s) -
Shigeru Miyagawa,
Ichiro Nakatsuka,
Yusuke Hayashi,
Hitoshi Higuchi,
Mikio Shimada,
Takuya Miyawaki
Publication year - 2008
Publication title -
neuropsychiatric disease and treatment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 67
eISSN - 1178-2021
pISSN - 1176-6328
DOI - 10.2147/ndt.s3063
Subject(s) - heme oxygenase , nitric oxide synthase , lipopolysaccharide , inflammation , medicine , nitric oxide , heme , pharmacology , oxidative stress , oxidative phosphorylation , biochemistry , enzyme , chemistry
Delirium occurs in 23% of sepsis patients, in which pro-inflammatory cytokines and nitric oxide are suggested to be involved. However, in animal experiments, even a subseptic dose of lipopolysaccharide (LPS) injection induces both pro-inflammatory cytokines and inducible nitric oxide synthase in the brain, suggesting that the brain oxidative reaction can be induced in the subseptic condition. Then, we evaluated the changes of heme oxygenase-1 (HO-1), a sensitive oxidative marker, as well as interleukin (IL)-1beta, IL-6, and inductible nitric oxide synthase (iNOS) mRNA in the hypothalamus and hippocampus of rats using real-time PCR after peripheral injection of LPS (2.0 mg/kg). As a result, these four kinds of mRNAs were induced significantly in both areas after LPS injection. These results suggest that peripheral inflammation induces an oxidative reaction in the brain, even if the inflammation is not lethal. It is also considered that several pathways are involved in brain HO-1 induction.