Open AccessAnalytical Performance Evaluation of Three Commercial Rapid Nucleic Acid Assays for SARS-CoV-2
Author(s) -
Jie Yi,
Xiao Han,
Ziyi Wang,
Yu Chen,
Yingchun Xu,
Jie Wu
Publication year - 2021
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s321227
Subject(s) - nucleic acid , reproducibility , detection limit , rna , biology , virology , covid-19 , assay sensitivity , chromatography , microbiology and biotechnology , nucleic acid detection , chemistry , biochemistry , medicine , infectious disease (medical specialty) , pathology , gene , alternative medicine , disease
To cope with the SARS-CoV-2 epidemic, several rapid nucleic acid assays have been approved for use, but the analytical performance has not been well evaluated. In this report, two key performance parameters, analytical sensitivity (limit of detection) and reproducibility, of three approved rapid nucleic acid assays were assessed using heat-inactivated SARS-CoV-2 culture supernatants quantified by digital PCR.