Open Access<p>Characterization of a Novel <em>mcr-8.2-</em>Bearing Plasmid in ST395 <em>Klebsiella pneumoniae</em> of Chicken Origin</p>
Author(s) -
Xiaorong Yang,
Kai Peng,
Zhang YuXia,
Li Liu,
Ruichao Li
Publication year - 2020
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s256544
Subject(s) - colistin , plasmid , klebsiella pneumoniae , minion , biology , context (archaeology) , gene , microbiology and biotechnology , mcr 1 , genetics , enterobacteriaceae , genome , antibiotics , escherichia coli , nanopore sequencing , paleontology
The emergence of mobile colistin resistance mcr genes undermines the efficacy of colistin as the last-resort drug for multi-drug resistance infections and constitutes a great public health concern. Plasmids play a critical role in the transmission of mcr genes among bacteria. One colistin-resistant Klebsiella pneumoniae strain of chicken origin was collected and analyzed by antimicrobial susceptibility testing, PCR, conjugation assay and S1-PFGE. Whole-genome sequencing (WGS) approach combining Illumina and MinION platforms was utilized to decipher the underlying colistin resistance mechanism and genetic context. A novel mcr-8.2 -bearing plasmid p2019036D-mcr8-345kb with 345 655 bp in size encoding various resistance genes including floR, sul1, aadA16, aadA2, bla CTX-M-27 , bla DHA-1 , tet (D), dfrA12 and qnrB4 was identified responsible for the colistin resistance phenotype. Plasmid comparison has shown that the mcr-8.2 -bearing plasmid differed from other reported plasmids positive for mcr-8.2 but shared the same core mcr-8.2 -bearing conserved region. This study demonstrates the emergence of mcr-8.2 -bearing K. pneumoniae of animal origin is a potential risk to humans.