Open Access<p>Next-generation-sequencing technology used for the detection of <em>Mycoplasma hominis</em> in renal cyst fluid: a case report</p>
Author(s) -
Xiao Nan,
Wei Gai,
Wei-Guo Hu,
Jianxing Li,
Yan Zhang,
Xiuying Zhao
Publication year - 2019
Publication title -
infection and drug resistance
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.033
H-Index - 39
ISSN - 1178-6973
DOI - 10.2147/idr.s198678
Subject(s) - azithromycin , medicine , mycoplasma , tigecycline , mycoplasma pneumoniae , clarithromycin , linezolid , cyst , microbiology and biotechnology , surgery , gastroenterology , biology , antibiotics , pneumonia , bacteria , vancomycin , helicobacter pylori , genetics , staphylococcus aureus
Background: Mycoplasma is an opportunistic pathogen causing both urogenital and extragenital infections. The lack of cell wall renders Mycoplasma difficult to culture and identify with ordinary methods. Next-generation sequencing (NGS) is a new technology helping a lot in the diagnosis of infective diseases. In this case, NGS played a key role in the diagnosis of Mycoplasma infection. Case presentation: A mid-aged man suffering from renal cyst underwent cyst incision followed by invasive treatments to eliminate hematoma caused by renal artery hemorrhage. After the cyst incision operation, the patient had a persistent high temperature. The persistent increase of blood neutrophile granulocyte count and C-reaction protein suggested an unresolved infection. The empirically chosen anti-infective agents were meropenem and linezolid since the ordinary bacterial cultures of surgical site drainage and blood yielded a negative result. At postoperation day (POD) 17, NGS result of his drainage clearly indicated the pathogen was Mycoplasma hominis . At POD 24, the drug sensitivity test showed resistance to quinolones, clarithromycin and erythromycin, but intermediate to azithromycin. Since then, the antimicrobial agents were changed into azithromycin and kept unchanged until the patient was fully recovered and discharged at POD 39. Conclusion: When the ordinary laboratory diagnostic methods failed, NGS diagnosis could reduce the hospitalization expenses and shorten the lengths of hospital stay.