Open AccessCo-stimulatory and co-inhibitory immune markers in solid tumors with MET alterations
Author(s) -
Karen L. Reckamp,
Jasmine A. McQuerry,
Isa Mambetsariev,
Rebecca Pharaon,
Susan E. Yost,
Jeremy Fricke,
Tamara Mirzapoiazova,
Raju Pillai,
Leonidas Arvanitis,
Ziad U. Khan,
Marwan Fakih,
Yuan Yuan,
Marianna Koczywas,
Erminia Massarelli,
Prakash Kulkarni,
Sumanta K. Pal,
Martin Sattler,
Andrea Bild,
Ravi Salgia
Publication year - 2021
Publication title -
future science oa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 23
ISSN - 2056-5623
DOI - 10.2144/fsoa-2020-0159
Subject(s) - cancer research , immune system , immune checkpoint , colorectal cancer , biology , exon , missense mutation , medicine , pathology , immunology , cancer , immunotherapy , mutation , gene , genetics
The implication of MET alterations in solid tumors and the immune microenvironment remains elusive. Formalin-fixed, paraffin-embedded samples of 21 patients with solid tumors harboring MET alterations were used for immunohistochemical staining. Extracted RNA was analyzed with the NanoString nCounter human PanCancer immune profiling panel (NanoString Technologies, Inc., WA, USA). Patients were diagnosed with lung (n = 10), breast (n = 5), genitourinary (n = 3) or colorectal cancer (n = 3). Eleven had a MET missense mutation, four had an exon 14 splice site mutation and six had MET amplification. CD6 , CCL19 , CD40LG , XCR1 , MAGEA1 , ATM and CCL19 genes were significantly differentially expressed in MET -altered cancers. MET alterations may have a role in various solid tumors as potential therapeutic targets and combination therapy candidates with immune checkpoint inhibitors.