Open AccessStem cells out of the bag: characterization of ex vivo expanded mesenchymal stromal cells for possible clinical use
Author(s) -
Sérgio Lopes,
Susana Roncón,
Filipa Bordalo,
Fátima Amado,
Sara Ferreira,
Ana Catarina Pinho,
Joana Vieira,
Altamiro CostaPereira
Publication year - 2020
Publication title -
future science oa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 23
ISSN - 2056-5623
DOI - 10.2144/fsoa-2019-0129
Subject(s) - mesenchymal stem cell , cd90 , ex vivo , immunophenotyping , stromal cell , regenerative medicine , bone marrow , peripheral blood mononuclear cell , cd44 , stem cell , biology , in vivo , immunology , cancer research , microbiology and biotechnology , cd34 , cell , in vitro , flow cytometry , biochemistry
Aim: Mesenchymal stromal cells (MSC) are a promising tool for cellular therapy and regenerative medicine. One major difficulty in establishing a MSC expansion protocol is the large volume of bone marrow (BM) required. We studied whether cells trapped within a collection bag and filter system could be considered as a source of MSC. Results: From the 20 BM collection bag and filter systems, we recovered an average of 1.68 × 10 8 mononuclear cells, which is the equivalent to 60 ml of filtered BM. Mononuclear cells were expanded ex vivo to 17 × 10 6 MSC, with purity shown by a CD44 + , CD105 + , CD90 + and CD73 + immunophenotype, a reduction of 20% proliferating cells in a mixed lymphocyte reaction and also the ability of adipocyte differentiation. Conclusion: Long-term MSC cultures were established from the usually discarded BM collection bag and filter, maintaining an appropriate phenotype and function, being suitable for both investigation and clinical settings.