Open AccessEffect of mismatch between types of viral nucleic acid and intended targets of extraction kits on polymerase chain reaction-based testing
Author(s) -
Jin-Ho Kim,
Hanjun Kim,
KyoungJin Yoon
Publication year - 2022
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2022-0006
Subject(s) - nucleic acid , dna , rna , polymerase chain reaction , reverse transcriptase , polymerase , rna extraction , microbiology and biotechnology , real time polymerase chain reaction , serial dilution , biology , reverse transcription polymerase chain reaction , dna extraction , chemistry , biochemistry , gene , messenger rna , medicine , alternative medicine , pathology
Lab personnel generally select an extraction kit based on the nucleic acid (NA) type of the target. This study investigated the effect of mismatch between the NA type of the target and the intended target NA of the extraction kit on the polymerase chain reaction outcome. DNA, RNA and total NA extraction kits manufactured by the same company were used to isolate NA from serial dilutions of four viruses representing different genome types. All extracts were tested for the viruses by either conventional or real-time polymerase chain reactions with and without reverse transcription. While the DNA kit specifically isolated DNA from samples, the RNA kit extracted both DNA and RNA as efficiently as the total NA kit, suggesting that RNA kits can be an economical alternative.