Open AccessEffective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites
Author(s) -
Dustin W Bailey,
Ziv Attia,
Stephan Reinert,
Brent S. Hulke,
Nolan C. Kane
Publication year - 2022
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2021-0050
Subject(s) - asteraceae , helianthus annuus , biology , sunflower , heliantheae , dna extraction , extraction (chemistry) , botany , dna , chromatography , chemistry , polymerase chain reaction , horticulture , biochemistry , gene
The Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites – specifically, phenolic compounds – on the extraction of DNA from Silphium spp. This genus is in the Heliantheae tribe of Asteraceae that also includes sunflower ( Helianthus annuus). Three methods were attempted in Silphium, with varying condition and age of the leaf sample. A modified cetyl trimethylammonium bromide (CTAB) method on young leaves resulted in the best DNA yield, with sufficient sample purity. No perceptible difference was observed between fresh and lyophilized samples for any extraction method or leaf age. These results provide an excellent basis for DNA extraction of difficult plant samples.