Open AccessEffects of a fully enclosed hollow-fiber centrifugal ultrafiltration technique for laboratory biosafety improvement
Author(s) -
Jing Yang,
Weigang Wang,
Cuixin Qiang,
Yanan Niu,
Zhirong Li,
MengQiang Zhao,
Ye Jiang,
Jian Cao,
Xiaoran Niu,
Xiaoxuan Liu,
Jianhong Zhao
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2021-0029
Subject(s) - biosafety , filtration (mathematics) , ultrafiltration (renal) , microbiology and biotechnology , biology , materials science , virology , chromatography , chemistry , mathematics , statistics
Laboratory biosafety has become a core focus in biological analysis, owing to the frequent occurrence of laboratory-acquired infections caused by the leakage of pathogenic microorganisms. For this purpose, the authors developed a safe pretreatment device combining a sealing technique with a direct injection technique. In this study, several bacteria and viruses were used to validate the filtration effect of the invention. Data show that the new device can completely filter bacteria and that the filtration rates for hepatitis B virus and hepatitis C virus reached 94% and 96%, respectively. The results show that the new preparation device can effectively block these pathogens and can improve biological safety and provide powerful protection for technicians.