Open AccessColonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis
Author(s) -
Rizwan Ahmad,
Balawant Kumar,
Raju Lama Tamang,
Wei Xu,
Geoffrey A. Talmon,
Aaron M. Mohs,
Punita Dhawan,
Amar B. Singh
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2020-0172
Subject(s) - metastasis , colorectal cancer , cancer , medicine , cancer research , tumor microenvironment , transplantation , lung , lung cancer , pathology
The conventional orthotopic/xenograft models or genetically engineered murine models of colon cancer (CRC) are limited in their scope for a true understanding of tumor growth, progression and eventual metastasis in its natural microenvironment. In the currently used murine models of CRC metastasis, the metastasis occurs primarily in the liver, though lung metastasis accounts for a significant proportion of CRC metastasis. There is an urgent need for a murine model of CRC, which not only allows tumor progression in the colonic mucosa but also metastasis of the lung. The authors describe a minimally invasive murine model of colon cancer progression that may be ideal for a wide range of applications, including evaluating gene function, microenvironment, cancer metastasis and therapeutic translational research.