Open AccessThe determination of cystatin C in biological samples via the surface plasmon resonance method
Author(s) -
Michal Lesňák,
Dominik Jursa,
Marek Miskay,
Helena Riedlova,
Karla Barčová,
Milan Adámek
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2020-0151
Subject(s) - biochip , surface plasmon resonance , cystatin c , biosensor , materials science , surface plasmon , sensitivity (control systems) , nanotechnology , chromatography , chemistry , biomedical engineering , plasmon , optoelectronics , medicine , electronic engineering , biochemistry , nanoparticle , renal function , engineering
Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.