Open AccessProtocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering
Author(s) -
Dar-Jen Hsieh,
Sivakumar Periasamy,
Ko-Chung Yen,
YiChun Yeh,
YunJu Chen,
Hung-Chou Wang,
Yih-Wen Tarng
Publication year - 2021
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2020-0141
Subject(s) - decellularization , tissue engineering , extracellular matrix , regenerative medicine , scaffold , regeneration (biology) , biomedical engineering , economic shortage , stem cell , microbiology and biotechnology , medicine , biology , linguistics , philosophy , government (linguistics)
Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and regenerative medicine applications. Traditional methods for decellularization often disrupt the 3D architecture and damage the structural integrity of the ECM scaffold. To efficiently obtain natural ECM scaffolds from animal tissues and organs with intact architecture, we have developed a platform decellularization process using supercritical CO 2 and tested its potential application in tissue engineering. A combination of human mesenchymal stem cells with a decellularized dermal matrix scaffold allowed complete regeneration of skin structure in a porcine full-thickness wound model.