Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform | Zendy

Sharon L. Truesdell | Zendy; Estee L. George | Zendy; Marnie M. Saunders | Zendy

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Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform

Author(s) -

Sharon L. Truesdell,

Estee L. George,

Marnie M. Saunders

Publication year - 2020

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/btn-2019-0115

Subject(s) - microbiology and biotechnology , bone remodeling , cell culture , bone resorption , polydimethylsiloxane , chemistry , resorption , bone cell , multicellular organism , biomedical engineering , cell , biology , nanotechnology , materials science , medicine , biochemistry , endocrinology , genetics

Our lab has developed a lab-on-a-chip platform for bone remodeling that enables long-term culturing of bone cells out to 7 weeks and serves as a foundation toward a multicellular organ-on-a-chip system. Here, we optimized culturing protocols for osteoblasts, osteoclasts and osteocytes within the lab-on-a-chip and performed functional activity assays for quantifying bone formation and resorption. We analyzed cell seeding densities, feeding schedules and time in culture as a basis for optimizing culturing protocols. Further, we addressed concerns of sterility, cytotoxicity and leakage during the extended culture period within the polydimethylsiloxane chip. This system provides a method for quantifying the soluble effects of mechanically stimulated osteocytes on bone remodeling (formation/resorption).

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