Open AccessCellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform
Author(s) -
Sharon L. Truesdell,
Estee L. George,
Marnie M. Saunders
Publication year - 2020
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2019-0115
Subject(s) - microbiology and biotechnology , cell culture , bone remodeling , bone resorption , polydimethylsiloxane , resorption , chemistry , bone cell , biomedical engineering , biology , medicine , endocrinology , genetics , organic chemistry
Our lab has developed a lab-on-a-chip platform for bone remodeling that enables long-term culturing of bone cells out to 7 weeks and serves as a foundation toward a multicellular organ-on-a-chip system. Here, we optimized culturing protocols for osteoblasts, osteoclasts and osteocytes within the lab-on-a-chip and performed functional activity assays for quantifying bone formation and resorption. We analyzed cell seeding densities, feeding schedules and time in culture as a basis for optimizing culturing protocols. Further, we addressed concerns of sterility, cytotoxicity and leakage during the extended culture period within the polydimethylsiloxane chip. This system provides a method for quantifying the soluble effects of mechanically stimulated osteocytes on bone remodeling (formation/resorption).