Open AccessFocused size selection of cell-free DNA samples for liquid biopsy applications that rely on next-generation sequencing
Author(s) -
Christopher K. Raymond
Publication year - 2019
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2019-0071
Subject(s) - dna sequencing , computational biology , dna , genomic dna , biology , liquid biopsy , cell free fetal dna , genomic library , dna nanoball sequencing , selection (genetic algorithm) , genetics , computer science , base sequence , pregnancy , fetus , cancer , artificial intelligence , prenatal diagnosis
Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require isolation of DNA fragments within a particular size range. In this Benchmark article, two related methods for size-selective DNA fragment enrichment are described.