Open AccessAssay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients
Author(s) -
Colin Reily,
Dana V. Rizk,
Bruce A. Julian,
Jan Novák
Publication year - 2018
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2018-0042
Subject(s) - helix pomatia , lectin , galactose , chemistry , biotin , nephropathy , biochemistry , biology , snail , endocrinology , diabetes mellitus , ecology
IgA nephropathy, the most common primary glomerulonephritis worldwide, is characterized by glomerular deposition of galactose-deficient IgA1 and elevated serum levels of this IgA1 glycoform. Current ELISA methods lack sensitivity to assess galactose deficiency using small amounts of IgA1, which limits studies in primary cells due to modest IgA1 production in isolated peripheral-blood lymphocytes.