Open AccessCataract-preventing contact lens for in vivo imaging of mouse retina
Author(s) -
Wataru Ikeda,
Tomoya Nakatani,
Akiyoshi Uemura
Publication year - 2018
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/btn-2018-0040
Subject(s) - retina , in vivo , contact lens , lens (geology) , retinal , preclinical imaging , confocal , two photon excitation microscopy , fundus (uterus) , confocal microscopy , biology , ophthalmology , microbiology and biotechnology , optics , neuroscience , medicine , fluorescence , physics
In vivo imaging of mouse retinas using two-photon or confocal laser scanning fluorescence microscopy is a powerful tool for time-lapse analyses of the dynamic movements of cell populations. However, acute and reversible opacification of the crystalline lenses of mouse eyes under anesthesia decreases the visibility of the ocular fundus. Therefore, we developed a customized contact lens for preventing cataract during continuous retinal imaging in anesthetized mice. This experimental approach will aid in the elucidation of cellular and molecular dynamics in the CNS under physiological and pathological conditions.