Open AccessCo-Transfected SV40 Origin of Replication Activates Expression from SV40 Promoterless Constructs
Author(s) -
A. P. F. Flint,
Štefan Kaluz,
Milota Kaluzová,
Leila Sheldrick,
Pat Fisher,
K. Derecka
Publication year - 1999
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/99274st02
Subject(s) - transfection , plasmid , biology , microbiology and biotechnology , origin of replication , dna , dna replication , virus , genetics , gene
Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) origin of replication (ori) by cotransfection in COS-7 cells with plasmids containing the SV40 ori. This effect has consequences for the use of such plasmids to control transfection efficiency.