High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay | Zendy

Phillip A. Morin | Zendy; Robert Saiz | Zendy; Atousa Monjazeb | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay

Author(s) -

Phillip A. Morin,

Robert Saiz,

Atousa Monjazeb

Publication year - 1999

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/99273rr02

Subject(s) - genotyping , exonuclease , biology , genotype , single nucleotide polymorphism , genetics , microbiology and biotechnology , polymorphism (computer science) , dna , gene , dna polymerase

Single nucleotide polymorphisms (SNPs) are the class of DNA variants that are most common in the genome. Technologies are needed that allow relatively highthroughput, high-quality genotyping of SNPs, with rapid assay development for individual SNPs of interest. We have implemented an accurate and high-throughput SNP genotyping system using a commercially available fluorescent 5′ exonuclease assay. Optimization of the assay system for low-volume reactions and low-probe concentrations has reduced assay costs by 75%. Using a simple assay optimization process, we successfully developed genotyping assays for 92% of assays attempted (309 out of 335) and have generated over 200 000 genotypes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research