Open AccessIncreasing DNA Transfer Efficiency by Temporary Inactivation of Host Restriction
Author(s) -
Robert A. Edwards,
R. Allen Helm,
Stanley Maloy
Publication year - 1999
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/99265st02
Subject(s) - electroporation , dna , biology , exogenous dna , host (biology) , escherichia coli , salmonella , bacteria , transduction (biophysics) , genetics , plasmid , microbiology and biotechnology , gene , biochemistry
E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequency DNA transfer. This technique might be readily applied to a wide range of bacteria to increase DNA transfer between strains and species.