Open AccessAgarose-Embedded Tissue Arrays for Histologic and Genetic Analysis
Author(s) -
Gladys S. Tsao-Wu,
Clifford H. Weber,
Lynn R. Budgeon,
Keith C. Cheng
Publication year - 1998
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/98254st02
Subject(s) - agarose , danio , zebrafish , biology , anatomy , microtome , morphological analysis , pathology , biomedical engineering , microbiology and biotechnology , computer science , genetics , gene , medicine , artificial intelligence
To facilitate the histologic analysis of large numbers of 7-day-old zebrafish (Danio rerio), a method has been developed to process them in agarose-embedded arrays. Using thin tissue sections, the morphology of cells and tissues can be examined microscopically to investigate a variety of biologic processes. Because of their small size, precise arrangement of the larvae is necessary to section them simultaneously. A technique was designed to embed groups of zebrafish larvae in a single plane in agarose before sectioning. Stained tissue sections of thousands of larvae can be examined efficiently using this embedding method. In addition to histologic analysis, PCR-based genotypic analysis of DNA from individual larval sections is also possible. This technique can be modified to accommodate any study that requires the histologic examination of many pieces of tissue.