Open AccessHigh-Temperature, Nonradioactive Primer Extension Assay for Determination of a Transcription-Initiation Site
Author(s) -
Mamoru Yamada,
Hanae Izu,
Takenori Nitta,
Kazunobu Kurihara,
Tamami Sakurai
Publication year - 1998
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/98251st02
Subject(s) - reverse transcriptase , primer extension , thermus aquaticus , primer (cosmetics) , thermus , microbiology and biotechnology , primer dimer , biology , rna , polymerase , dna , chemistry , polymerase chain reaction , biochemistry , enzyme , base sequence , multiplex polymerase chain reaction , gene , thermophile , organic chemistry
We have developed a simple and safe method for the determination of a transcription-initiation site. In this method, reverse transcriptase of the avian myeloblastosis virus or rTth DNA polymerase from Thermus thermopilus was used with a fluorescein isothiocyanate (FITC)-labeled primer. The primer-extension reaction can be performed at a high temperature, which reduces the hindering effect of the secondary structure in RNA, and can omit the annealing step between RNA and the primer. Almost all steps can be done in one tube. This procedure can provide reliable and reproducible data when compared with the conventional procedure at low temperature. Moreover, the sequencing ladder that is required for determining the position of extended products can be obtained with the same FITC-labeled primer.