Open AccessPhosphorylation-Directed Antibodies in High-Flux Screens for Compounds that Modulate Signal Transduction
Author(s) -
John A. Alberta,
Charles D. Stiles
Publication year - 1997
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/97233st07
Subject(s) - phosphopeptide , phosphorylation , signal transduction , tyrosine phosphorylation , biology , protein phosphorylation , antibody , receptor , biochemistry , context (archaeology) , tyrosine , microbiology and biotechnology , computational biology , protein kinase a , genetics , paleontology
Synthetic tyrosine phosphopeptides were used to generate antibodies that recognize phosphotyrosine in the context of a defined sequence of flanking amino acids. Using phosphopeptide immunogens derived from regulatory or signal-generating motifs, “phosphorylation-directed antibodies” can be targeted to specific growth factor receptors or signal-generating proteins. In this paper, we show how phosphorylation-directed antibodies can be used in a colorimetric, high-throughput screen for drugs that modulate the function of specific growth factor receptors or signal-generating proteins.