Phosphorylation-Directed Antibodies in High-Flux Screens for Compounds that Modulate Signal Transduction | Zendy

John A. Alberta | Zendy; Charles D. Stiles | Zendy

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Phosphorylation-Directed Antibodies in High-Flux Screens for Compounds that Modulate Signal Transduction

Author(s) -

John A. Alberta,

Charles D. Stiles

Publication year - 1997

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/97233st07

Subject(s) - phosphopeptide , phosphorylation , signal transduction , tyrosine phosphorylation , biology , protein phosphorylation , antibody , receptor , biochemistry , context (archaeology) , tyrosine , microbiology and biotechnology , computational biology , protein kinase a , genetics , paleontology

Synthetic tyrosine phosphopeptides were used to generate antibodies that recognize phosphotyrosine in the context of a defined sequence of flanking amino acids. Using phosphopeptide immunogens derived from regulatory or signal-generating motifs, “phosphorylation-directed antibodies” can be targeted to specific growth factor receptors or signal-generating proteins. In this paper, we show how phosphorylation-directed antibodies can be used in a colorimetric, high-throughput screen for drugs that modulate the function of specific growth factor receptors or signal-generating proteins.

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