Open AccessPCR-Amplified cDNA Probes for Verification of Differentially Expressed Genes
Author(s) -
Ralf Ross,
Katja Kumpf,
Angelika B. ReskeKunz
Publication year - 1997
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/97225st01
Subject(s) - complementary dna , biology , gene , differential display , gene expression , microbiology and biotechnology , genetics , computational biology
Differential display has proven to be a powerful technique for the detection and isolation of differentially expressed genes. By generating reproducible cDNA expression patterns, it is possible to compare gene expression by two or more cell types, developmental stages or tissues and to isolate as yet unknown differentially expressed genes. A sensitive method is necessary to verify the differential expression of the isolated cDNAs. Here we describe the use of adaptor-ligated, PCR-amplified total cDNA of the two cell types compared as a probe for Southern hybridizations with the isolated cDNAs.