Open AccessNovel Oxygenation System Supports Multilayer Growth of HeLa Cells
Author(s) -
Catherine Rappaport,
Edward M. Trujillo,
Lih-Farn Soong
Publication year - 1996
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/96214st05
Subject(s) - hela , gelatin , microbiology and biotechnology , biophysics , cell , adhesion , in vitro , cell adhesion , biology , mitosis , in situ , staining , chemistry , anatomy , biochemistry , genetics , organic chemistry
We have developed a novel substratum in which gelatin is bonded to a reservoir of perfluorodecalin using a perfluoroalkylating technique. This forms a stable substratum supporting good adhesion for cells. HeLa cells cultured on this substratum continued to grow exponentially after the surface was covered with a monolayer forming a tissue-like structure of more than 19 layers of cells. Histological sectioning and staining of the block of tissue formed revealed the presence of mitotic figures deep within the structure. Every cell was surrounded by other cells, similar to growth of cells in vivo. This technique opens up a new approach to studying problems involved in cell-cell interaction and development of histotypic structures in vitro.