Comparison of Plasmid DNA Preparation Methods for Direct Gene Transfer and Genetic Immunization | Zendy

Heather L. Davis | Zendy; Martin Schleef | Zendy; Peter Moritz | Zendy; M Mancini | Zendy; Joachim Schorr | Zendy; Robert G. Whalen | Zendy

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Comparison of Plasmid DNA Preparation Methods for Direct Gene Transfer and Genetic Immunization

Author(s) -

Heather L. Davis,

Martin Schleef,

Peter Moritz,

M Mancini,

Joachim Schorr,

Robert G. Whalen

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96211st03

Subject(s) - plasmid , gene , dna , transfection , biology , reporter gene , microbiology and biotechnology , immunization , genetics , dna vaccination , gene expression , antigen

Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.

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