Open AccessComparison of Plasmid DNA Preparation Methods for Direct Gene Transfer and Genetic Immunization
Author(s) -
Heather L. Davis,
Martin Schleef,
Peter Moritz,
Maryline Mancini,
Joachim Schorr,
Robert G. Whalen
Publication year - 1996
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/96211st03
Subject(s) - plasmid , gene , dna , transfection , biology , reporter gene , microbiology and biotechnology , immunization , genetics , dna vaccination , gene expression , antigen
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.