Open AccessSelection of Murine Lymphoid and Hematopoietic Cells Using Polystyrene Tissue Culture Devices Containing Covalently Immobilized Antibody
Author(s) -
Amy E. Berson,
Kirsten Catharina Christina Knobel,
Debra Rood,
K. Chen,
Donald Lamons,
Maureen A. Mcnally,
Thomas B. Okarma,
Jane Lebkowski
Publication year - 1996
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/96206pf02
Subject(s) - bone marrow , haematopoiesis , microbiology and biotechnology , antibody , progenitor cell , biology , cell culture , antigen , stem cell , immunology , genetics
We have established rapid procedures that negatively deplete and positively select for specific murine cell populations. By using polystyrene tissue culture flasks containing a covalently bound mouse anti-rat antibody and specific anti-mouse, cell-surface antigen antibodies, we easily and efficiently depleted greater than 90% of the mature lineage cells from murine bone marrow. This selection procedure resulted in an enrichment of progenitor colonies (CFU-Cs) in murine bone marrow. Using the same polystyrene tissue culture devices, we can directly isolate CD117 + (c-kit + ) murine hematopoietic cells. As few as 2000 of these CD117 + cells rescued and reconstituted lethally irradiated recipients in a murine bone marrow transplant model.