Selection of Murine Lymphoid and Hematopoietic Cells Using Polystyrene Tissue Culture Devices Containing Covalently Immobilized Antibody | Zendy

Amy E. Berson | Zendy; K.M. Knobel | Zendy; Debra Rood | Zendy; KE Chen | Zendy; Donald Lamons | Zendy; Maureen A. McNally | Zendy; T B Okarma | Zendy; Jane Lebkowski | Zendy

Open Access

Selection of Murine Lymphoid and Hematopoietic Cells Using Polystyrene Tissue Culture Devices Containing Covalently Immobilized Antibody

Author(s) -

Amy E. Berson,

K.M. Knobel,

Debra Rood,

KE Chen,

Donald Lamons,

Maureen A. McNally,

T B Okarma,

Jane Lebkowski

Publication year - 1996

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/96206pf02

Subject(s) - bone marrow , haematopoiesis , microbiology and biotechnology , antibody , progenitor cell , biology , cell culture , antigen , stem cell , immunology , genetics

We have established rapid procedures that negatively deplete and positively select for specific murine cell populations. By using polystyrene tissue culture flasks containing a covalently bound mouse anti-rat antibody and specific anti-mouse, cell-surface antigen antibodies, we easily and efficiently depleted greater than 90% of the mature lineage cells from murine bone marrow. This selection procedure resulted in an enrichment of progenitor colonies (CFU-Cs) in murine bone marrow. Using the same polystyrene tissue culture devices, we can directly isolate CD117 + (c-kit + ) murine hematopoietic cells. As few as 2000 of these CD117 + cells rescued and reconstituted lethally irradiated recipients in a murine bone marrow transplant model.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research