Open AccessTaqMan®-based quantification of invasive cells in the chick embryo metastasis assay
Author(s) -
Edward Htun van der Horst,
Jörg Hendrik Leupold,
Rainer Schubbert,
Axel Ullrich,
Heike Allgayer
Publication year - 2004
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/04376st02
Subject(s) - taqman , embryo , biology , microbiology and biotechnology , metastasis , polymerase chain reaction , genetics , gene , cancer
The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan ® technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range.