Open AccessDevelopment of multiplex DNA electronic microarrays using a universal adaptor system for detection of single nucleotide polymorphisms
Author(s) -
Shirley Tsang,
Zhaogang Sun,
Claudia Stewart,
Nicole Lum,
Casey Frankenberger,
Marianne Subleski,
Lynn Rasmussen,
David J. Munroe
Publication year - 2004
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/04364dd02
Subject(s) - multiplex , dna microarray , snp genotyping , oligonucleotide , genotyping , computational biology , biology , microarray , genetics , single nucleotide polymorphism , microbiology and biotechnology , dna , genotype , gene , gene expression
The NanoChip ® electronic microarray is designed for the rapid detection of genetic variation in research and clinical diagnosis. We have developed a multiplex electronic microarray assay, specific for single nucleotide polymorphism (SNP) genotyping and mutation detection, using universal adaptor sequences tailed to the 5′ end of PCR primers specific to each target. PCR products, amplified by primers directed to the universal adaptor sequence, are immobilized on the microarray either directly or via capture oligonucleotides complementary to the universal adaptor sequence. This simple modification results in a significant increase in fidelity with improved specificity and accuracy. In addition, the multiplexing of genetic variant detection allows increased throughput and significantly reduced cost per assay. This general schema can also be applied to other microarray and macroarray formats.