Method for Linking a Synthesized Protein to Its mRNA-DNA Complex | Zendy

Shumo Liu | Zendy; G. V. Shivashankar | Zendy; Tohru Sano | Zendy; Albert Libchaber | Zendy

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Method for Linking a Synthesized Protein to Its mRNA-DNA Complex

Author(s) -

Shumo Liu,

G. V. Shivashankar,

Tohru Sano,

Albert Libchaber

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00294st06

Subject(s) - messenger rna , luciferase , microbiology and biotechnology , dna , transcription (linguistics) , biology , ribosome , translation (biology) , protein biosynthesis , gene , genetics , rna , transfection , linguistics , philosophy

A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should stay in a complex with its mRNA and DNA. A method is designed to test these possibilities. Using an immobilized luciferase gene sequence, a functional luciferase protein is produced that remains associated through its mRNA with its DNA, confirming the feasibility of the proposed scheme. It has potential application for in vitro synthesis of proteins and protein micro-arrays.

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