Open AccessSingle step BP/LR combined Gateway reactions
Author(s) -
Xiquan Liang,
Lansha Peng,
Chang-Ho Baek,
Federico Katzen
Publication year - 2013
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114101
Subject(s) - clone (java method) , cloning (programming) , gateway (web page) , biology , computational biology , dna , genetics , exploit , molecular cloning , computer science , gene , peptide sequence , computer security , world wide web , programming language
The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.