Single Step BP/LR Combined Gateway Reactions | Zendy

Xiquan Liang | Zendy; Lansha Peng | Zendy; Chang-Ho Baek | Zendy; Federico Katzen | Zendy

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Single Step BP/LR Combined Gateway Reactions

Author(s) -

Xiquan Liang,

Lansha Peng,

Chang-Ho Baek,

Federico Katzen

Publication year - 2013

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114101

Subject(s) - clone (java method) , cloning (programming) , gateway (web page) , biology , computational biology , dna , genetics , exploit , molecular cloning , computer science , gene , peptide sequence , computer security , world wide web , programming language

The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.

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