Open AccessLow-cost media formulation for culture of brain tumor spheroids (neurospheres)
Author(s) -
Michael D. Monterey,
Nicholas Szerlip,
Saroj P. Mathupala
Publication year - 2013
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114066
Subject(s) - spheroid , neurosphere , brain tumor , cell culture , explant culture , glioblastoma , tumor cells , primary culture , biology , cancer research , immunology , pathology , in vitro , biochemistry , medicine , genetics , endothelial stem cell , adult stem cell
Recent studies have found that the biological features of primary tumors are faithfully recapitulated when a patient's tumor is processed and then maintained as a 3-D spheroid in specialized cell culture media. However, a major drawback for maintenance and routine passage of primary tumors as spheroids has been the high cost of custom-formulated media compared to regular serum-supplemented media. Here we report the formulation of a cost-effective, serum-free medium in which high-grade primary brain tumor (glioblastoma) explants can be established and maintained as spheroids. Based on DMEM, this formulation requires only supplementation with several amino acids, vitamins, synthetic EGF, and bFGF, with most of the cost being associated with the growth factors. A simple addition of BSA (fraction V) obviated the need for numerous other components (or human serum) commonly used in the specialized commercial media formulations.