Open AccessProduction of single-stranded DNAs by self-cleavage of rolling-circle amplification products
Author(s) -
Hongzhou Gu,
Ronald R. Breaker
Publication year - 2013
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114009
Subject(s) - deoxyribozyme , rolling circle replication , dna , cleavage (geology) , encode , template , oligonucleotide , nucleic acid , biology , chemistry , nanotechnology , genetics , gene , materials science , dna replication , paleontology , fracture (geology)
DNA molecules that encode a small, high-speed self-hydrolyzing deoxyribozyme are used as templates for rolling circle amplification (RCA) to produce single-stranded DNAs (ssDNAs) of single- and multiple-unit lengths. Including self-cleaving deoxyribozymes in RCA products can generate large amounts of ssDNAs with defined sequence and length as well as precise termini. We also demonstrate the use of this method to efficiently generate ssDNA size markers by using deoxyribozyme reaction conditions that permit partial processing.