Open AccessZeocin for selection of bleMX6 resistance in fission yeast
Author(s) -
Zsigmond Benkő,
Yuqi Zhao
Publication year - 2011
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000113706
Subject(s) - auxotrophy , biology , complementation , selection (genetic algorithm) , yeast , genetics , schizosaccharomyces pombe , fission , gene , microbiology and biotechnology , saccharomyces cerevisiae , mutant , physics , computer science , quantum mechanics , artificial intelligence , neutron
Complementation of auxotrophic nutrient deficiencies in minimal media is widely used for selection of exogenous gene introduction to fission yeast. However, only a limited number of such selection markers are available. Antibiotic resistance markers are good alternatives, but they typically work well in complete rich medium but not in minimal defined Edinburgh minimal medium (EMM). It would be ideal if both the auxotrophic and antibiotic resistance markers can be used together for molecular genetic analysis. Here we describe the use of Zeocin in Pombe minimal glutamate (PMG) media for selection and maintenance of bleMX6 resistance with a LEU2 auxotrophic marker in fission yeast.