Open AccessA simplified, 96-well–adapted, ATP luminescence–based motility assay
Author(s) -
Audrey Restouin,
Sandra Aresta,
Thomas Prébet,
Jean-Paul Borg,
Ali Badache,
Yves Collette
Publication year - 2009
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000113250
Subject(s) - motility , luminescence , microbiology and biotechnology , biology , chemistry , physics , optoelectronics
Directional motility assays make use of Boyden chambers or Transwell culture inserts with porous membranes that separate cells seeded in the upper chamber from a chemoattractant supplied in a lower chamber. These assays are often time-consuming and are associated with several limitations due to manual counting and inconsistent results; low signal-to-noise ratio and fluorescence interference; and high cost and the need for specific equipment. Here, we describe a simple, direct, and easy ATP luminescence–based motility assay (ALMA), which can be used for 96-well plate quantification.