A simplified, 96-well–adapted, ATP luminescence–based motility assay | Zendy

Audrey Restouin | Zendy; Sandra Aresta | Zendy; Thomas Prébet | Zendy; JeanPaul Borg | Zendy; Ali Badache | Zendy; Yves Collette | Zendy

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A simplified, 96-well–adapted, ATP luminescence–based motility assay

Author(s) -

Audrey Restouin,

Sandra Aresta,

Thomas Prébet,

JeanPaul Borg,

Ali Badache,

Yves Collette

Publication year - 2009

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113250

Subject(s) - motility , chemotaxis , luminescence , membrane , fluorescence , plate reader , signal (programming language) , biophysics , chemistry , microbiology and biotechnology , chromatography , biology , biochemistry , materials science , optics , computer science , optoelectronics , physics , receptor , programming language

Directional motility assays make use of Boyden chambers or Transwell culture inserts with porous membranes that separate cells seeded in the upper chamber from a chemoattractant supplied in a lower chamber. These assays are often time-consuming and are associated with several limitations due to manual counting and inconsistent results; low signal-to-noise ratio and fluorescence interference; and high cost and the need for specific equipment. Here, we describe a simple, direct, and easy ATP luminescence–based motility assay (ALMA), which can be used for 96-well plate quantification.

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