An Automated Home-Built Low-Cost Fermenter Suitable for Large-Scale Bacterial Expression of Proteins in Escherichia Coli | Zendy

Uwe Riek | Zendy; Roland Tuerk | Zendy; Theo Wallimann | Zendy; Uwe Schlattner | Zendy; Dietbert Neumann | Zendy

Open Access

An Automated Home-Built Low-Cost Fermenter Suitable for Large-Scale Bacterial Expression of Proteins in Escherichia Coli

Author(s) -

Uwe Riek,

Roland Tuerk,

Theo Wallimann,

Uwe Schlattner,

Dietbert Neumann

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112830

Subject(s) - industrial fermentation , escherichia coli , bacteria , fermentation , recombinant dna , pellet , protein expression , yield (engineering) , genetically engineered , biology , biomass (ecology) , bacterial growth , chromatography , chemistry , food science , microbiology and biotechnology , biochemistry , materials science , gene , genetics , zoology , agronomy , metallurgy

We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.

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