Open AccessFlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco
Author(s) -
José M. Estévez,
Chris Somerville
Publication year - 2006
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000112264
Subject(s) - arabidopsis , green fluorescent protein , biology , gene , live cell imaging , microbiology and biotechnology , fluorescence microscope , plant cell , fluorescence , cell , biochemistry , mutant , physics , quantum mechanics
Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter. Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT 2 reagents.