Flash-Based Live-Cell Fluorescent Imaging of Synthetic Peptides Expressed in Arabidopsis and Tobacco | Zendy

José M. Estevez | Zendy; Chris Somerville | Zendy

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Flash-Based Live-Cell Fluorescent Imaging of Synthetic Peptides Expressed in Arabidopsis and Tobacco

Author(s) -

José M. Estevez,

Chris Somerville

Publication year - 2006

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112264

Subject(s) - arabidopsis , biology , green fluorescent protein , live cell imaging , gene , microbiology and biotechnology , cell , biochemistry , mutant

Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter. Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT 2 reagents.

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