Open AccessMulti-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown
Author(s) -
Daqian Sun,
Margherita Melegari,
Sunandini Sridhar,
Charles E. Rogler,
Liang Zhu
Publication year - 2006
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000112203
Subject(s) - gene knockdown , microrna , gene , biology , computational biology , rna interference , small hairpin rna , genetics , regulation of gene expression , rna
A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.