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Identification of Formate‐Metabolizing Bacteria in Paddy Soil by DNA‐Based Stable Isotope Probing
Author(s) -
Feng Youzhi,
Lin Xiangui,
Jia Zhongjun,
Zhu Jianguo
Publication year - 2012
Publication title -
soil science society of america journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.836
H-Index - 168
eISSN - 1435-0661
pISSN - 0361-5995
DOI - 10.2136/sssaj2011.0216
Subject(s) - formate , bacteria , stable isotope probing , ralstonia , organic matter , chemistry , oryza sativa , microorganism , food science , microcosm , biology , environmental chemistry , botany , biochemistry , organic chemistry , gene , catalysis , genetics
As one of the fermentation products of plant biomass, formate is an important organic acid in flooded rice ( Oryza sativa L.) fields. Little is known, however, about the identity of formate‐metabolizing bacteria in paddy soil. In this study, a paddy soil microcosm was incubated with 13 C‐labeled formate; bacterial and eukaryotic communities incorporating formate C were analyzed by DNA‐stable isotope probing. Real‐time quantitative polymerase chain reaction analysis revealed that 13 C‐formate‐derived C was actively assimilated by a subset of bacteria. Molecular fingerprinting and sequencing analysis of 16S rRNA genes revealed that the active formate‐metabolizing bacteria in the paddy soil were mainly the bacteria in the Clostridia class and purple nonsulfur bacteria (PNSB). The former might facilitate SO 4 2− and Fe reduction using formate. Observed for the first time, PNSB are able to assimilate organic matter for growth under in situ conditions without light. These results extend our knowledge of not only the formate‐assimilating bacteria but also the ecological significance of formate in paddy soil.